atcc 66981 (ATCC)

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ATCC atcc 66981
Atcc 66981, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a+alternata+atcc+66981/Alternaria+alternata+(Fries)+Keissler/10__1016_slash_j__biocontrol__2025__105947-115-14-14
Average 95 stars, based on 83 article reviews

atcc 66981 - by Bioz Stars, 2026-09

95/100 stars

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Sequencing:

Article Title: Whole-genome sequencing and de novo assembly of Alternaria alternata ATCC 66981.
Article Snippet: .. There were 29 BGCs predicted within the genome sequence of A. alternata ATCC 66981 distributed as follows: seven T1PKS clusters, three terpene clusters, five NRPS clusters, six fungal-RiPP-like clusters, one NAPAA cluster, and six NRPS-like clusters. ..

Article Title: Whole-genome sequencing and de novo assembly of Alternaria alternata ATCC 66981
Article Snippet: .. There were 29 BGCs predicted within the genome sequence of A. alternata ATCC 66981 distributed as follows: seven T1PKS clusters, three terpene clusters, five NRPS clusters, six fungal-RiPP-like clusters, one NAPAA cluster, and six NRPS-like clusters. ..

Article Title: Identification, mycotoxigenic ability and biosynthesis genes of Alternaria spp. from apples in China
Article Snippet: .. Using the genome sequence of A. alternata ATCC 66981 as a reference genome for splicing, PacBio subreads (length ≥ 1 kb) were self-correction with the program Canu v1.5 (parameters: estn = 24, npruseGrid = 0, corOvlMemory = 4; https://github.com/marbl/canu/releases). .. Corrected reads were assembled using Falcon Version v0.3.0 (parameters: -v -dal8 -t32 -h60 -e.96 -l500 -s100 -H3000; https://github.com/PacificBiosciences/f alco), and single-base corrections with GATK v1.6–13 (parameters: -cluster 2 -window 5 -stand_call_conf 50 -stand_emit_conf 10.0 -dcov 200 MQ0 ≥ 4; http://www.broadinstitute.org/gatk).

Protein-Protein interactions:

Article Title: Identification, mycotoxigenic ability and biosynthesis genes of Alternaria spp. from apples in China
Article Snippet: The heatmaps of biosynthesis genes in the clusters were visualized using the Morpheus tool (Morpheus (broadinstitute.org)). .. The protein sequences and the protein-protein interaction (PPI) network of A. alternata ATCC 66981 were retrieved from STRING (version 11.5, https://string-db.org/), with a minimum required interaction score of 0.4. ..

Cell Culture:

Article Title: Effects of Essential Oil Citral on the Growth, Mycotoxin Biosynthesis and Transcriptomic Profile of Alternaria alternata
Article Snippet: .. A. alternata ATCC 66981 was acquired from American Type Culture Collection and cultured on Potato Dextrose Agar (PDA) for 6 days at 25 °C. ..

Article Title: Transcriptomic Insights into the Antifungal Effects of Magnolol on the Growth and Mycotoxin Production of Alternaria alternata
Article Snippet: .. RNA was extracted from 6 days cultured mycelia from A. alternata ATCC 66981 grown in PDB medium with or without 25 μM of magnolol using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s instructions. ..

Control:

Article Title: Transcriptomic Insights into the Antifungal Effects of Magnolol on the Growth and Mycotoxin Production of Alternaria alternata
Article Snippet: .. Figure S1: Volcano plot of these differentially expressed genes (DEGs) of A. alternata treated with 25 μM of magnolol vs. control, Figure S2: Comparative analysis of gene expression of A. alternata in 0, 12.5, 25 μM of magnolol by quantitative reverse transcription PCR (qRT-PCR), Table S1: Summary of the RNA-Seq data in the control (AaC) and 25.0 μM magnolol treated (AaM) groups of A. alternata ATCC 66981, Table S2: Differentially expressed genes (DEGs) between the control and magnolol stressed samples of A. alternata . ..

Gene Expression:

Reverse Transcription:

Polymerase Chain Reaction:

Transformation Assay:

Article Title: Establishment of CRISPR/Cas9 in Alternaria alternata.
Article Snippet: The filamentous fungus Alternaria alternata is a potent producer of many secondary metabolites, some of which like alternariol or alternariol-methyl ether are toxic and/or cancerogenic.. Many Alternaria species do not only cause post-harvest losses of food and feed, but are aggressive plant pathogens.. Despite the great economic importance and the large number of research groups working with the fungus, the molecular toolbox is rather underdeveloped.

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